USDA CENTRAL SMALL GRAIN GENOTYPING LAB, MANHATTAN KS
New Arrival: We now have a new semi-automatic DNA extraction robot, the Oktopure, from LGC Genomics. This robot uses a proprietary magnetic bead based extraction chemistry and can extract eight 96-well plates of samples in about 3 hours. We plan to use this DNA for all future projects.
- Single Nucleotide Polymorphism Tightly Linked to a Major QTL on Chromosome 7A for Both Kernel Length and Kernel Weight in Wheat
- Single Nucleotide Polymorphisms Linked to Quantitative Trait Loci for Grain Quality Traits in Wheat
- Using Next Generation Sequencing for Multiplexed Trait-Linked Markers in Wheat
- Identification of markers linked to genes for sprouting tolerance (independent of grain color) in hard white winter wheat.pdf
- Mapping quantitative trait loci for plant adaptation and morphology traits in wheat using single nucleotide polymorphisms
- Independent mis-splicing mutations in TaPHS1 causing loss of preharvest sprouting (PHS) resistance during wheat domestication
- Single nucleotide polymorphism markers linked to QTL for wheat yield traits
- Genotyping-by-sequencing (GBS) identified SNP tightly linked to QTL for pre-harvest sprouting resistance
Current Research Projects
- Improving Barley and Wheat Germplasm for Changing Environments
- Development of Abiotic Stress-Resistant Germplasm
- Development of High-Throughput Markers for Genetic Improvement of Wheat for Multiple Traits
- Genetic Improvement of Hard Red Winter Wheat to Biotic and Abiotic Stresses
Goals and Objectives
The goals of the USDA Central Small Grain Genotyping Center in Kansas:
1. Conduct research to accelerate the breeding process by identifying novel DNA markers and genes for important wheat traits.
2. Use markers to select desired wheat genotypes.
3. Provide feedback to breeders seeking to create new cultivars in response to specific challenges such as newly emergent plant pathogens or changing global weather patterns.
Our specific objectives:
1. Maximize the efficiency of plant breeding programs by applying high-throughput DNA marker-assisted selection (MAS) technology, resulting in the early release of superior germplasm and cultivars.
2. Develop new and robust DNA markers associated with resistance to biotic and abiotic stresses and end use quality in wheat through next generation sequencing and functional gene cloning.
3. Fingerprint a core set of cultivars and their breeding parents with DNA markers to develop molecular marker profiles for these cultivars, which will be cross-linked to other genetic information currently available in other USDA databases.
4. Exploit new technologies such as next generation sequencing and other high throughput genotyping technologies for gene discovery and genomic selection in breeding programs.
5. Provide training and consultation on marker analysis to breeders and other scientists.