USDA CENTRAL SMALL GRAIN GENOTYPING LAB, MANHATTAN KS
LAB ACTIVITY HALTED DUE TO CORONAVIRUS
Our lab on the campus of Kansas State University has halted all normal activity and projects due to the coronavirus pandemic. In person activity for both the USDA and KSU is currently prohibited. Only essential and emergency personnel are allowed on campus at this time. All personnel are working from home via telework protocols and are still reachable by email.
Multiplex Restriction Amplicon Sequencing (MRASeq)
We have developed a novel, rapid, inexpensive, patent-free, next-generation sequencing-based platform for marker discovery, genotyping, and mapping, comparable to genotyping-by-sequencing (GBS), which can be used in quantitative trait loci (QTL) studies, background selection in breeding, and many other genetics and breeding applications in any species (Plant Biotechnology Journal, 2019).
Physical maps showing the random distribution of 7,595 sMRASeq markers (a) and the nonrandom distribution of 7,564 GBS markers (b) in a wheat population.
Resistance to Fusarium Head Blight in Wheat is Controlled by the TaHRC Gene
Published June 10th, 2019 in Nature Genetics, we have discovered that resistance to fusarium head blight in wheat is controlled by the TaHRC gene.
Two RNAi transgenic (T1) 'Bobwhite' plants show resistance to FHB spread within a spike compared with the non-transgenic 'Bobwhite' 8 days after inoculation. A diagram of the gene structure of TaHRC showing upstream untranslated regions (gray bars), the 25 bp deletion in the ORF of TaHRC-R (red bar), and the start (ATG) and stop (TAA) codons, and sequence comparison of the start codon region of TaHRC across six susceptible wheat genotypes and the resistant wheat 'Ning'.
The USDA and Kansas State University both have issued press releases about this discovery. Here are the USDA press release and the KSU press release.
- Multiplex restriction amplicon sequencing a novel next-generation sequencing-based marker platform for high-throughput genotyping, 2019
- A deletion mutation in TaHRC confers Fhb1 resistance to Fusarium head blight in wheat, 2019
- Thatcher wheat line RL6149 carries Lr64 and a second leaf rust resistance gene on chromosome 1DS, 2019
- Fine Mapping of the Wheat Leaf Rust Resistance Gene Lr42, 2019
- Agronomic Performance of Hard Red Winter Wheat Lines Introgressed With the Fhb1 Gene, 2019
- Registration of ‘Matterhorn’ Hard White Waxy Winter Wheat, 2019
- Persistence of rye (Secale cereale L.) chromosome arm 1RS in wheat (Triticum aestivum L.) breeding programs of the Great Plains of North America, 2019
- Biofortification of Hard Red Winter Wheat by Genes Conditioning Low Phytate and High Grain Protein Concentration, 2019
- Development and validation of diagnostic markers for Fhb1 region, a major QTL for Fusarium head blight resistance in wheat, 2018
Current Research Projects
- Improving Barley and Wheat Germplasm for Changing Environments
- Development of Abiotic Stress-Resistant Germplasm
- Development of High-Throughput Markers for Genetic Improvement of Wheat for Multiple Traits
- Genetic Improvement of Hard Red Winter Wheat to Biotic and Abiotic Stresses
Goals and Objectives
The goals of the USDA Central Small Grain Genotyping Center in Kansas:
1. Conduct research to accelerate the breeding process by identifying novel DNA markers and genes for important wheat traits.
2. Use markers to select desired wheat genotypes.
3. Provide feedback to breeders seeking to create new cultivars in response to specific challenges such as newly emergent plant pathogens or changing global weather patterns.
Our specific objectives:
1. Maximize the efficiency of plant breeding programs by applying high-throughput DNA marker-assisted selection (MAS) technology, resulting in the early release of superior germplasm and cultivars.
2. Develop new and robust DNA markers associated with resistance to biotic and abiotic stresses and end use quality in wheat through next generation sequencing and functional gene cloning.
3. Fingerprint a core set of cultivars and their breeding parents with DNA markers to develop molecular marker profiles for these cultivars, which will be cross-linked to other genetic information currently available in other USDA databases.
4. Exploit new technologies such as next generation sequencing and other high throughput genotyping technologies for gene discovery and genomic selection in breeding programs.
5. Provide training and consultation on marker analysis to breeders and other scientists.