USDA CENTRAL SMALL GRAIN GENOTYPING LAB, MANHATTAN KS
LAB ACTIVITY REDUCED DUE TO CORONAVIRUS
Our lab on the campus of Kansas State University has limited normal activity and projects due to the coronavirus pandemic. In person activity is currently allowed, but limits on the number of persons per room are still active. All personnel are still reachable by email.
Identification of Candidate Chromosome Region of (Sbwm1) for Soil-Borne Wheat Mosaic Virus Resistance in Wheat
Using association analysis, the Sbwm1 gene has been located within a 620 kb region on chromosome 5D. The candidate region includes several disease resistance related genes and we identified a PTI1-like tyrosine-protein kinase 1 gene as a putative candidate gene for Sbwm1. Two flanking SNPs for Sbwm1 have been converted into KASP assays and can effectively separate resistant and susceptible lines. (Nature: Scientific Reports, 2020).
Manhattan plots for wheat resistance to Soil-borne wheat mosaic virus (SBWMV) in an association mapping population (A) and the physical positions of the significant SNPs on chromosome 5D (B). The red line represents the threshold for significant SNPs.
- Identification of candidate chromosome region of sbwm1 for soil-borne wheat mosaic virus resistance in wheat, 2020
- Increased prediction accuracy using combined genomic information and physiological traits in a soft wheat panel evaluated in multi-environments, 2020
- Identification of two novel Hessian fly resistance genes H35 and H36 in a hard winter wheat line SD06165, 2020
- The Hessian fly recessive resistance gene H4 mapped to chromosome 1A of the wheat cultivar ‘Java’ using genotyping‐by‐sequencing, 2020
- Genetic dissection of heat-responsive physiological traits to improve adaptation and increase yield potential in soft winter wheat, 2020
- Registration of 'Bobcat' hard red winter wheat, 2020
- Multiplex restriction amplicon sequencing a novel next-generation sequencing-based marker platform for high-throughput genotyping, 2019
- A deletion mutation in TaHRC confers Fhb1 resistance to Fusarium head blight in wheat, 2019
Current Research Projects
- Improving Barley and Wheat Germplasm for Changing Environments
- Development of Abiotic Stress-Resistant Germplasm
- Development of High-Throughput Markers for Genetic Improvement of Wheat for Multiple Traits
- Genetic Improvement of Hard Red Winter Wheat to Biotic and Abiotic Stresses
Goals and Objectives
The goals of the USDA Central Small Grain Genotyping Center in Kansas:
1. Conduct research to accelerate the breeding process by identifying novel DNA markers and genes for important wheat traits.
2. Use markers to select desired wheat genotypes.
3. Provide feedback to breeders seeking to create new cultivars in response to specific challenges such as newly emergent plant pathogens or changing global weather patterns.
Our specific objectives:
1. Maximize the efficiency of plant breeding programs by applying high-throughput DNA marker-assisted selection (MAS) technology, resulting in the early release of superior germplasm and cultivars.
2. Develop new and robust DNA markers associated with resistance to biotic and abiotic stresses and end use quality in wheat through next generation sequencing and functional gene cloning.
3. Fingerprint a core set of cultivars and their breeding parents with DNA markers to develop molecular marker profiles for these cultivars, which will be cross-linked to other genetic information currently available in other USDA databases.
4. Exploit new technologies such as next generation sequencing and other high throughput genotyping technologies for gene discovery and genomic selection in breeding programs.
5. Provide training and consultation on marker analysis to breeders and other scientists.